Phenotypic Characterization and Comparative Genomic Analyses of Mycobacteriophage WIVsmall as A New Member Assigned to F1 Subcluster

In this study, we conducted the morphological observation, biological and genomic characterization, evolutionary analysis, comparative genomics description, proteome identification of a recently isolated mycobacteriophage, WIVsmall. Morphologically, WIVsmall is classified as member Siphoviridae family, characterized by flexible tail, measuring approximately 212 nm in length. The double-stranded phage genome DNA spans 53,359 base pairs, exhibits G + C content 61.01%. comprises 103 protein-coding genes, while no tRNA genes were detected. annotation unveiled presence functional gene clusters responsible for mycobacteriophage assembly maturation, replication, cell lysis, protein synthesis. Based on analysis phylogenetic tree, was belonging to subgroup F1. A indicated that exhibited highest similarity SG4, with percentage 64%. single-step growth curve revealed latent period 120 min, an outbreak 200 min.